Automated procedure for assay of 2,3-diphosphoglycerate in red cells by measuring enzymatically released inorganic phosphorus.
نویسندگان
چکیده
The recent awareness of the importance of DPG’ in the metabolism of red blood cells has heightened interest in methods of analysis for that compound. Concentrations of DPG in red cells are increased in conditions of hypoxia produced at high altitudes, or by chronic lung disease, congenital heart diseases, or anemia (1). Also, the oxygen-delivering capability of stored banked blood seems to be improved when the DPG concentrations are maintained (2) or restored (3). Rose and Liebowitz (4) observed that DPG phosphatase activity of muscle PGM is stimulated greatly by phosphoglycolic acid.
منابع مشابه
Reduced Red Cell 2 , 3 - Diphosphoglycerate and Adenosine
Serum inorganic phosphorous was decreased significantly on the third postoperative day following cardiac surgery in 18 patients initially studied. Reduced plasma inorganic phosphate has been shown to cause a reduced concentration of red cell organic phosphates, an important determinant of hemoglobin-oxygen affinity. Therefore, 10 consecutive patients were studied to determine if reduced 2,3-dip...
متن کاملMeasurement of adenosine triphosphate and some other metabolites in blood cells by isotachophoresis. II. Application to monitoring changes during storage in acid-citrate-dextrose preservative.
The applicability and accuracy of isotachophoresis in measuring the concentrations of some of the intermediates of red cell metabolism during blood bank storage in acid-citrate-dextrose preservative was assessed. Preparative treatment consisted of washing with isotonic sucrose solution and centrifugation to remove serum, but white cells and platelets were not separated out from red blood cells....
متن کاملReduced red cell 2,3-diphosphoglycerate and adenosine triphosphate, hypophosphatemia, and increased hemoglobin-oxygen affinity after cardiac surgery.
Serum inorganic phosphorous was decreased significantly on the third postoperative day following cardiac surgery in 18 patients initially studied. Reduced plasma inorganic phosphate has been shown to cause a reduced concentration of red cell organic phosphates, an important determinant of hemoglobin-oxygen affinity. Therefore, 10 consecutive patients were studied to determine if reduced 2,3-dip...
متن کاملA phosphorus-31 nuclear magnetic resonance investigation of intracellular environment in human normal and sickle cell blood.
Intracellular pH and 2,3-diphosphoglycerate concentration in sickle cell amenia and normal human blood samples were measured by means of phosphorus-31 nuclear magnetic resonance spectroscopy. To monitor the concentrations of various internal phosphorylated metabolites of intact red blood cells, heparinized blood samples were used and were incubated at 37 degrees C with 5.6% C92, 25% O2, and 69....
متن کاملMeasurement of adenosine triphosphate and some other metabolites in blood cells by isotachophoresis. I. Preparative technique and enzymatic confirmation.
This study investigated the optimal conditions for detection of nucleotides in blood using an IP-1B capillary isotachophoretic apparatus. The system used 10 mM HCl-beta-alanine (pH 4.2) as the leading electrolyte and n-caproic acid as the terminal electrolyte. Direct application of lysed red blood cells was shown to be inaccurate, and a method of deproteinization based on heat in a microwave ov...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 18 11 شماره
صفحات -
تاریخ انتشار 1972